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biological molecules, on the photoirradiated area The provision of an immobilization process is one of the most essential processing steps that is required to obtain practical biomolecule carriers such as biosensors, bioreactors, or biochips Therefore, a large number of immobilization techniques have already been developed for biological molecules, in which the molecules are immobilized on a carrier using covalent bonds (Feng et al, 2005), ionic bonds (Lee et al, 2003), physical adsorption (Ouyang et al, 2003), cross-linkage of the biomolecules (Levy and Shoseyov, 2004), or microencapsulation (Hartmann, 2005) Chemically induced immobilization methods require optimized processes depending on the structures and properties of the individual biomolecules, which in turn require some complicated procedures (Peluso et al 2003); yet these techniques are widely used Azopolymers can immobilize microobjects that possess a variety of surface characteristics, including negatively charged DNA, charged proteins, and hydrophobic polystyrene.

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The characteristics of the azopolymer make it possible to immobilize a wide variety of biological molecules on the same substrate through a one-step photoirradiation process To take advantage of the functionality of these biomolecules, identifying an immobilization process that does not lead to deactivation of the molecules is important In particular, biomolecules such as proteins show sensitive behavior in terms of changes in environment, as shown by the denaturing of proteins when the surrounding temperature increases even slightly Since it is possible that damage to biomolecules following photoinduced immobilization could trigger functional degradation, the authors rst examined the activity of an immobilized enzyme An aqueous solution of 1-mg/mL bacterial protease (subtilisin; 275 kDa, Sigma) was spotted onto the surface of an azopolymer, and the surface was irradiated with a laser beam of 488-nm wavelength and 80-mW/cm2 optical power density for 5 min to immobilize the enzyme.

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are introduced, then (1.4.30) The protolytic reaction between two molecules must be formulated as the formation of the pair B + HA^BH+A~ (1.4.31) that can react as a base with another acid HA,, or as an acid with another base B n : B,H + Ar + HA n <P* B,H + A,r + HA, BjH+Ar + B n ^ B u H + A r + Bj ' '

As a control experiment, a similar specimen was prepared without photoirradiation The activity of the subtilisin was veri ed as the hydrolysis of the arti cial substrate (tert-butoxycarbonyl-Gly-Gly-Leu-p-nitroanilide, Mw=4655, Merck) The arti cial substrate solution was spotted onto the azopolymer surface in the same area where the subtilisin had been immobilized, and then the specimen was maintained at 37 1C and 85% relative humidity for 1 h The hydrolysis of the arti cial substrate was determined spectroscopically by immediately measuring the absorbance of the reactant at a wavelength of 410 nm The conversion ratio of the reaction was B10% for the subtilisin-immobilized sample, whereas it was B1% for the control sample (without photoirradiation) These results clearly show that biomolecules immobilized on an azopolymer surface can maintain their enzyme functionality during and after the immobilization process.

approach yields even greater benefits by allowing multiple users to run on a single blade (either PC blade or server blade), increasing utilization, and reducing the number of blades needed to support the user base. Although the PC blade market has traditionally not been included in industry-analyst statistics, a significant number of users are operating on PC blades. The benefits of blade technology, coupled with the advances in virtualization in this area, make desktops a large growth opportunity, particularly with the evolution of the virtual desktop. This chapter completes the architectural look at the computing portion of blade technology. The next two chapters examine the I/O aspects of blade server systems and take a closer look at architectural issues and considerations for networking and storage in a blade environment.

Protolytic reactions can also occur in fused salts. The solvent participates in these reactions provided that at least one of its ions has protogenic and/or protophilic character. An example of a solvent in which the cation is aprotic and the anion protophilic is ethylpyridinium bromide (m.p. 114 C). The acid HA is protolysed in this solvent (HA + B r ~ ^ H B r + A"). Hydrogen bromide acts as a solvated proton and the acidity is expressed as

Next, the authors investigated how deformation of an azopolymer surface can be induced by biomolecules as well as by microspheres A phosphate-buffered saline (PBS) solution containing Cy-5-linked immunoglobulin, IgG, was spotted onto the surface of an azopolymer After evaporating the solution, the surface was irradiated for 30 min with light of 470-nm wavelength and 10-mW/cm2 optical power density from an array of blue light-emitting diodes (LEDs) and then the.