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and 2-nm deep can be observed on the surface. In contrast, no dents were formed on the azopolymer surface where no IgG was deposited. Comparing these images, the dents formed on the surface in Fig. 9.9b are considered to mirror the surface shape of the IgG. These ndings lead to the conclusion that the azopolymer surface recognizes each molecular shape and deforms along the contours of the biomolecules during photoirradiation, as shown in Fig. 9.9b. The results also suggest that the increase in contact area between the azopolymer and biomolecules after photoirradiation restrains desorption from the surface. As described later, the authors also examined the possibility of antigen antibody reactions on the surface of the azopolymers. PBS solutions of human serum albumin and bovine serum albumin with different concentrations were spotted onto azopolymer surfaces according to the layout shown in Fig. 9.10a. After evaporating the spotted solution, photoirradiation was performed over the entire surface using an array of blue LEDs and was then washed with PBS containing 0.01 wt% Tween 20 to remove the un-immobilized albumins. After drying the sample again, the obtained sample was reacted with anti-HSA monoclonal mouse antibodies, and then the washed sample was reacted with Cy-5-labeled

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61 some of substances exhibit only one of the features required, whilst lacking others, and thus their placing in the acid or base group is not certain The concept of 'hard' and 'soft' acids and bases ('HSAB') should also be mentioned here This is not a new theory of acids and bases but represents a useful classification of Lewis acids and bases from the point of view of their reactivity, as introduced by R G Pearson Hard acids (HA) have low polarizability and small dimensions, higher oxidation numbers and the 'hardness' increases with increasing oxidation number; they bind bases primarily through ionic bonds Typical examples are H + , Na + , Hg 2+ , Ca 2+ , Sn2+, VO 2+ , VO 2 2+ , (CH3)2Sn2+, A1(CH3)3, I 7+ , I 5+ , Cl 7+ , CO2, R3C+, SO3.

1 Photoirradiation BSA HSA BSA HSA (a) 0.5

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Soft acids (SA) are strongly polarizable, have low or zero oxidation numbers, the 'softness' decreases with increasing oxidation number and if groups with a large negative charge are also bonded to the central atom, then the 'softness' increases; they bind bases primarily through covalent bonds Typical examples are Cu + , Ag + , Hg + , Cs + , Pd 2+ , Cd2+, Hg 2+ , Tl 3+ , T1(CH3)3, BH3, I + , Br + , HO 2 + , I2, Br2, O, Cl, Br, I, metal atoms, metals in the solid phase Hard bases (HB) have low polarizability and strong Br0nsted basicity; in the V, VI and VII groups of the periodic table, the first atom is always the hardest and the hardness decreases with increasing atomic number in the group.

To provide this information-delivery function, networks must meet the following basic requirements of a transport system: error-free delivery, highspeed movement, flexibility in quality of service, flexible media and signals, pathway virtualization, and high availability.

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Typical examples are H2O, OH~, F", O 2 ", CH3COO~, SO42~, Cl", CO32", NO 3 ", ROH, RCT, R2O, NH3, RNH2, N2H4 Soft bases (SB) are strongly polarizable and generally have low Br0nsted basicity Typical examples are R2S, RSH, RS", I~, R3P, CN", CO, QH4, C6H6, H , R Obviously, a number of substances cannot be classified exactly Borderline acids include Fe 2+ , Sb 3+ , Ir 3+ , B(CH3)3, SO2, NO + , R 3 C + and C6H5+ and borderline bases include C6H5NH2, N3~, Br~, NO 2 ", and SO2~ Simple laws govern the reactions of these substances: hard acids react preferentially with hard bases and soft acids with soft bases Mixing of 'unsymmetrical' complexes results in the reaction HA SB + SA HB-^ HA HB + SA SB (14.

0.01 0.001 0.0005 ( g)

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34) For example, the ether RO C^ (of the HB SA type) is split by hydrogen iodide (of the HA SB type) to yield ROH (the HB HA type) and I G^ (the SB SA type) It is obvious that the Lewis theory and the HSAB concept are very important for the description of the mechanisms of chemical reactions; however, for electrochemistry the Br0nsted theory is quite adequate 145 Correlation of the properties of electrolytes in various solvents.

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