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62 actually true if acids or bases are compared in a single solvent For example, in aqueous solutions values of KA of 138 x 1(T3, 174 x 10~5 and 427 x 10~7 have been found for chloroacetic acid, acetic acid and carbonic acid, respectively; consequently, in (dilute) aqueous solutions chloroacetic acid is two orders of magnitude stronger than acetic acid which is again two orders stronger than carbonic acid These values have no absolute significance They change on transfer to another solvent and even their order can change If, for example, the protolytic reaction CH3COOH + H2O < * = CH3COO~ + H 3 O + occurred in acetic acid medium, with water as a dissolved base, then the standard state to which the activities and equilibrium constants are related would be changed Comparison of the acidity or basicity of solvents is just as difficult.

In the next phase, the authors applied the photoimmobilization method to try to obtain protein chips, and more speci cally, an immunochip Enzyme-linked immunosorbent assay (ELISA) systems are commonly used (Karlsson et al, 1991; Voller et al, 1978) as a popular method for detecting small amounts of protein in sample solutions such as serums Although the ELISA system is an excellent method for detection of proteins, it still has some problems; it is comparatively expensive, and it is dif cult to detect proteins when small quantities of the sample solution are used The authors might be able to realize an immunochip that could act as a micro-ELISA system with the capability to deal with small quantities of sample solutions if they could succeed in immobilizing the antibodies on the substrate.

For example, alcohols react according to the equation ROH +H 3 O + ^ ROH 2 + + H2O In dilute solutions (water concentration of less than 1 mol dm" 3 ), the constant K' for this reaction in methanol is equal to 86 x 10~3 and in ethanol, 39 x 10~3 If these values were also valid in the vicinity of composition [ROH]/[H2O] = 1, then the ratio [ROH2+]/[H3O+] = /T[ROH]/[H2O] describing the proton distribution between the alcohol and water would be small Consequently, water would have a greater affinity for protons than alcohol, ie would be more basic The opposite is true The effect of the medium (solvent) on the dissolved substance can best be expressed thermodynamically Consider a solution of a given substance (subscript i) in solvent s and in another solvent r taken as a reference.

Networks today can operate at gigabit rates through their transceiver send/ receive functions implemented in hardware. For various technologies, these rates include 1, 2, 4, 8, 10, 16, and 20 gigabits per second. Gigabit rates allow networks to represent a bit of information in much less time from server to server and server to storage. High-speed options are available in various forms with GbE, Fibre Channel, and InfiniBand, with varying configurations and adapters continuing to come into the marketplace over time.

Such an immunochip could be used to measure multiple target proteins on the same substrate simultaneously, so it has the potential to become a novel method of replacing conventional ELISA systems in the elds of biochemical indexes, diagnostic agents, and clinical inspection (Kambhampati, 2003) One can say that the photoimmobilization method is one of the most promising prospects for immunochip applications because it can provide immobilization on the substrate surface irrespective of the surface states of the biomolecules The authors rst examined speci c reactions of photoimmobilized antibodies on azopolymer surfaces for the immunochip application Solutions of anti-goat antibodies (left-hand side) and anti-rabbit antibodies (right-hand side) were spotted onto an azopolymer surface at different concentrations, the layout of which is shown in Fig 911a After photoimmobilization and washing, the sample was reacted separately with Cy-5-labeled antigens (goat IgG and rabbit IgG).