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In recent years, polymer brushes have become fascinating subjects in polymer research. They are de ned as dense layers of end-grafted polymer chains con ned to a solid surface or interface (Advincula et al., 2004). Recently, well-de ned polymer brush structures with a high graft density have been developed using living radical polymerization, such as the atomic transfer radical polymerization (ATRP) method (Tsujii et al., 2006; Edmondoson et al., 2004). Since the ATRP can be performed using various types of monomers, many functional polymer brushes can be designed. Introduction of LC properties into the surface-graft chains seems of great interest in view of fabrication of smart responsive surfaces (Hamelinck and Huck, 2005; Peng et al., 1999). Uekusa et al. (2007) have synthesized LC polymer brushes bearing an Az mesogenic group in the side group by adopting the surfaceinitiated ATRP method. The polymer brushes have characteristic properties quite different and unpredictable from those of the spincast lms. A unique orientation behavior of a smectic LC state in the thin lms prepared by the surface-initiated polymerization is indicated. UV-visible absorption spectroscopic measurements reveal that the Az mesogens adopt a parallel orientation in the lm, which is in sharp contrast to that in a spincast lm of the identical polymer. A normal orientation of the mesogenic groups is commonly observed for the spincast lms of smectic LC Az polymer (Han et al., 2000, 1999; Geue et al., 1997).

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Hoekstra, Membrane fusion: from liposomes to biological membranes, Trends Biochem ScL, 9, 479 (1984) 65 651 Examples of Biological Membrane Processes Processes in the cells of excitable tissues.

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A 2-D XRD pro le of the grafted polymer lm recorded on an imaging plate is depicted in the top portion of Fig. 8.6. Diffraction patterns are observed only in the in-plane direction, that is, the periodic structures derived from the smectic LC phase are perpendicular to the substrate plane with LC mesogens being aligned parallel to the substrate. An in-plane orientation of mesogens is often observed in a microphase-separated state of LC block copolymers (Mao et al., 1997); however, to our knowledge, this type of smectic LC orientation in a polymer lm was observed for the rst time. In the one-dimensional (1-D) pattern, a clear peak is observed at 2y=2.481, which corresponds to a layer spacing of 3.56 nm. This spacing corresponds to a long-range ordering of approximately the length of the fully extended Az side chain, suggesting the formation of interdigitated structures of Az side groups. In contrast, a diffraction pattern on the spincast lm of the identical homopolymer is observed only in the out-of-plane direction at 2y=2.731 (d=3.26 nm) (Fig. 8.6, bottom). On the basis of the spectroscopic and X-ray data, schematic illustrations of the structure of the surface-grafted and spincast polymer lm are shown in Fig. 8.7.

The transport of information from sensors to the central nervous system and of instructions from the central nervous system to the various organs occurs through electric impulses transported by nerve cells (see Fig. 6.17). These cells consist of a body with star-like projections and a long fibrous tail called an axon. While in some molluscs the whole membrane is in contact with the intercellular liquid, in other animals it is covered with a multiple myeline layer which is interrupted in definite segments (nodes of Ranvier). The Na+,K+-ATPase located in the membrane maintains marked ionic concentration differences in the nerve cell and in the intercellular liquid. For example, the squid axon contains 0.05 MNa+, 0.4 M K + , 0.04-0.1 MC1~, 0.27 M isethionate anion and 0.075 M aspartic acid anion, while the intercellular liquid contains 0.46 M Na + , 0.01 M K+ and 0.054 M Cl". The relationship between the electrical excitation of the axon and the membrane potential was clarified by A. L. Hodgkin and A. P. Huxley in