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Azoderivatized gramicidin ion channels represent a unique case where ion transport can be photocontrolled by the optical manipulation of a biomolecule (Lien et al, 1996) In addition to obvious applications in controlled transport, this offers the possibility of studying cells by controlling the timing of ion exchange processes Photoinduced catalysis is also possible, for instance, using molecules where only the cis form is catalytically active (Wuerthner and Rebek, 1995) Extension of the molecular imprinting technique to azo polymers allows for photoswitching of binding activity with respect to the imprinted molecule (Minoura et al, 2004) 1262 Photoprobes The properties of an azo chromophore (spectrum, isomerization kinetics, etc) depend strongly on the local environment This enables the possibility of using the chromophore as a molecular sensing element: a photoprobe.

systems. Transitioning from rack servers to blades might mean that time needed to deploy shrinks from two weeks down to days or even hours. Additionally, blades and virtualization software make it easy to reprovision servers for use by another application or group of users, allowing resources to be reallocated based on business needs such as faster response to unexpected spikes in traffic.

Table 6.2 Apparent formal redox potentials of systems present in the electrontransfer chain (pH = 7). It should be noted that the potential values were obtained in the homogeneous phase. Due to stabilization in a membrane, the oxidationreduction properties vary so that the data listed below are of orientation character only Oxidized form Cytochrome a3 (ox) Cytochrome c (ox) Cytochrome b (ox) Coenzyme Q Flavinadenindinucleotide (FAD) Fumarate Nicotinamide adenindinucleotide (NAD+) Reduced form

For instance, it has been found that many azo properties depend on local H+ concentration, to the extent that the azo can in fact be used as a pH meter (Uznanski and Pecherz, 2002; Mermut and Barrett, 2001) As mentioned earlier, the isomerization kinetics can also be used as a probe of free volume (Naito et al, 1993; Lamarre and Sung, 1983), local aggregation (Norman and Barrett, 2002), or phase transitions The azo molecule is small and exhibits clean photochemistry, which makes it more versatile and robust than many other photoprobes The rate of isomerization is also remarkably insensitive to temperature (Yamamoto, 1986), yet sensitive to local solvent conditions (Li et al, 2006; Norman and Barrett, 2002) This is an area of research that deserves considerably more attention In a more sophisticated example, azo chromophores were used to monitor protein folding (Bredenbeck et al.

All of the management benefits described here can be translated (directly or indirectly) into reduced OpEx. These include everything from initial deployment to adding blades to reconfiguring and reprovisioning. Additionally, having complete remote-management ability eliminates travel in cases other than hardware replacement, while allowing management to be done centrally even when blade systems are geographically distributed. As one user put it, Our staff was reduced due to a slump in business, and we are able to support hundreds of virtual servers with just a few people.

'(pH = 7)(V)

, 2003; Sporlein et al, 2002) Speci cally, femtosecond 2-D IR spectroscopy was used to monitor the distances between carbonyl groups in the peptide An azo chromophore, incorporated inside the polypeptide chain, was used as a photoswitch to initiate a conformational change, hence initiate protein folding, on demand Combined with time-resolved monitoring of the azo spectrum, this allows the deconvolution of folding dynamics Pumpprobe ultrafast laser pulse experiments are being used to study many different chemical reactions, but are obviously limited to reactions that can be triggered by light Incorporating azobenzene into the experiment allows a wider range of reactions to be phototriggered..

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Finally, the simplicity and manageability of blade systems and virtualization solutions changes the level of expertise of the repair staff. Since everything except for a hardware swap can be done remotely, when there is a hardware failure on a blade, virtualization software can automatically restart the VM on another blade (high availability), keeping the applications running. The blade hardware swap requires little to no technical expertise. It is said that even the Federal Express person could swap out a failing blade (and then ship it to the appropriate location for repair).

1263 Optical Data Storage The azos have been investigated as optical storage media for some time Early proofs of principle were on Langmuir Blodgett lms, using photochromism (Liu et al, 1990) or birefringence (Dhanabalan et al, 1999) Increasingly, amorphous polymer systems are being recognized as promising materials In these easily processed systems, the birefringence is strong, stable, and switchable, making them ideal for optical memories A single domain could encode one bit by either being isotropic or birefringent, a difference that is easily probed optically The Dn values are large enough, in fact, that a gray-level algorithm could be used, where each domain stores more than one bit of data On the negative side, the photoalignment generated in the direction of the read write beam leads to an effective loss of material performance with time.

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